Institution
Carnegie Institution for Science
Open Date
June 8, 2022
Location
Stanford, CA, USA

Description
We are looking for a talented recent graduate to work as a Research Assistant (RA) in the laboratory of Dr. Sue Rhee at the Carnegie Institution for Science, Department of Plant Biology, located in Stanford, California. Our lab is generally interested in uncovering the molecular mechanisms of plant adaptation to environmental stresses. We work with different organisms ranging from model plant Arabidopsis thaliana to economically important crops such as Sorghum bicolor, an economically important bioenergy crop. We are looking for RA to work on the Sorghum Metabolic Atlas project. Our goal is to create a high-resolution cellular map of sorghum metabolic enzymes through deciphering enzyme localization within the cell. Information about enzyme localization contributes to the current understanding of enzyme functions and metabolic networks. We use a variety of cellular and molecular biology techniques to identify enzyme subcellular localization. The RA will be assisting a postdoc to scale up the scope of this project by conducting enzyme localization experiments from start-to-finish, which includes growing and maintaining plants, Gateway cloning to generate destination vectors, transient expression of enzymes followed by identifying localization through confocal microscopy.

We are looking for a highly motivated, organized and experienced recent graduate with a BS or BA degree in biology, cellular and molecular biology or related fields. The position is particularly relevant for students seeking more laboratory research experience before applying to graduate school. Experience in basic molecular biology techniques, including but not limited to, PCR amplification, cloning, plasmid isolation, and bacterial transformation is required. Knowledge about plant transient transformation is a plus. Previous experience in growing and maintaining plants is recommended.

Institution
Duke University, Biology
Location
Durham, NC, USA
Closing date
November 12, 2021

Description
The Benfey lab is a leading developmental biology lab located at Duke University. We are seeking to hire a full-time technician who will primarily work on a project examining single cell gene expression in the model plant Arabidopsis thaliana. Duties will include preparation of single cell RNA-Sequencing libraries, as well as tasks related to general molecular biology, and use/maintenance of a state of the art light sheet microscope. Training will be provided. This is a position suitable for a recent graduate seeking to obtain lab experience before graduate school, or anyone interested in working on a project using cutting edge technologies to help answer fundamental questions on developmental biology. Bachelor's degree in life sciences related field and experience in a molecular biology lab are required.

Institution
Carnegie Institution for Science
Location
Stanford, CA, USA
Earliest starting date
October 1, 2021
Closing date
Flexible, accepting applications until filled. Will start assessing applications on September 1, 2021.

Description
The Burlacot lab is opening on October 1, 2021 at the Department of Plant Biology of the Carnegie Institution for Science. We will start a scientific journey in the study of the molecular mechanisms that allow photosynthesis to be the major CO2 cycling mechanism in the atmosphere. We are seeking a molecular biology and algal physiology Lab Technician to help set up the lab and start new research programs in algal biology. We aim to recruit highly motivated and creative people with strong training in microbiology and molecular biology.

Our lab aims to understand the acclimation of photosynthesis to environmental fluctuations and the dynamics of the molecular mechanisms involved. The questions that our lab aims to address are: How are microalgae dynamically reacting to environmental fluctuations? How do molecular mechanisms of photosynthesis dynamically interact to optimize cell survival and growth under fluctuating conditions? How do the different time scales of photosynthetic energy transfers combine at various time scales of light fluctuations? What genes are important for acclimation to such fluctuations? Can we modify key enzymes to make photosynthesis more robust to some environments?

Qualifications

• Bachelor degree in microbiology, Plant science, cell biology or a related field.
• Good skills in microbiology and molecular biology.
• Basic lab skills, preparing solutions, and sterile technique.
• Proficiency in MS Word, Excel and Powerpoint required.
• Willingness to take responsibilities in a small team and to actively contribute to develop the lab organisation.
• Willingness to take part in building a dynamic, inclusive and interactive lab atmosphere.
• Willingness to develop synergistic interactions with other labs from the Carnegie Institution for Science and from Stanford University.
• Programming skills: ability to develop and implement simple codes in Python and/or Mathlab will be appreciated.
• Prior experience with microalgae or plants is a plus but is not necessary.