Research Assistant (Rhee Lab)

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Institution
Carnegie Institution for Science
Open Date
June 8, 2022
Location
Stanford, CA, USA

Description
We are looking for a talented recent graduate to work as a Research Assistant (RA) in the laboratory of Dr. Sue Rhee at the Carnegie Institution for Science, Department of Plant Biology, located in Stanford, California. Our lab is generally interested in uncovering the molecular mechanisms of plant adaptation to environmental stresses. We work with different organisms ranging from model plant Arabidopsis thaliana to economically important crops such as Sorghum bicolor, an economically important bioenergy crop. We are looking for RA to work on the Sorghum Metabolic Atlas project. Our goal is to create a high-resolution cellular map of sorghum metabolic enzymes through deciphering enzyme localization within the cell. Information about enzyme localization contributes to the current understanding of enzyme functions and metabolic networks. We use a variety of cellular and molecular biology techniques to identify enzyme subcellular localization. The RA will be assisting a postdoc to scale up the scope of this project by conducting enzyme localization experiments from start-to-finish, which includes growing and maintaining plants, Gateway cloning to generate destination vectors, transient expression of enzymes followed by identifying localization through confocal microscopy.

We are looking for a highly motivated, organized and experienced recent graduate with a BS or BA degree in biology, cellular and molecular biology or related fields. The position is particularly relevant for students seeking more laboratory research experience before applying to graduate school. Experience in basic molecular biology techniques, including but not limited to, PCR amplification, cloning, plasmid isolation, and bacterial transformation is required. Knowledge about plant transient transformation is a plus. Previous experience in growing and maintaining plants is recommended.