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Plant Cell Atlas Spatial Proteomics ​Workshop
Spatial Proteomics in Plants: How to map the location and connection of proteins

Virtual Workshop
April 8, 2021

 

Objectives

Registration


The goal of the Plant Cell Atlas (PCA) is to bring together a community who will  comprehensively describe plant cell types by integrating high-resolution subcellular and cellular location information of nucleic acids, proteins, and metabolites. In line with achieving this goal, proteomics represents a central facet of the systems-level landscape. Significant advances in mass spectrometry technologies and proteomic workflows now make proteomics notably more accessible for plant science. This PCA workshop aims to bring together world leaders in spatial and quantitative proteomics from plant sciences and beyond to help realize the development of a comprehensive PCA proteome.

Workshop Organizers and Speakers

Registration for the PCA Spatial Proteomics Workshop is now closed.

A Zoom link will be provided to registrants the day before the workshop.


You can find recordings of four of the talks on the Plant Cell Atlas YouTube Channel. Links to the individual talks can be found in the agenda.

Workshop Times
PDT - 8:00am - 11:30am
MDT - 9:00am - 12:30pm
CDT - 10:00am - 1:30pm
EDT - 11:00am - 2:30pm
CEST - 5:00pm - 8:30pm
GMT+8 - 11:00pm - 2:30am (4/9/2021)

Epidermal plasma membrane in green and nuclei (H2B-TFP) in blue, together with chlorophyll autofluorescence in red below in the mesophyll. Photo credit: Kate Harline (Roeder Lab)
Epidermal plasma membrane in green and nuclei (H2B-TFP) in blue, together with chlorophyll autofluorescence in red below in the mesophyll. Photo credit: Kate Harline (Roeder Lab)
Pin1-YFP (yellow) and DR5::RFP (red) in the maize shoot apex. Photo credit: Leiboff Lab
Pin1-YFP (yellow) and DR5::RFP (red) in the maize shoot apex. Photo credit: Leiboff Lab
Depth-coded image of microtubules in root hairs of the model grass, Brachypodium distachyon. Photo credit: Elison Blancaflor
 

Agenda

8:00 AM - 8:05 AM PDT
Introduction
8:05 AM - 8:25 AM PDT
Kathryn Lilley (University of Cambridge)
Talk Title: Spatial proteomics methods to map subcellular protein distribution and re-localisation
8:30 AM - 8:50 AM PDT
Ryan Kelly (Brigham Young University)
Talk Title: In-depth and high-spatial-resolution proteome profiling of plant tissues
8:55 AM - 9:15 AM PDT
Julia Mergner (Technical University of Munich)
Talk Title: Protein expression profiling in Arabidopsis tissues
9:20 AM - 9:30 AM PDT
Break
9:30 AM - 9:50 AM PDT
Nikolai Slavov (Northeastern University)
Talk Title: Mapping the transcriptome and proteome of tissues in 3D at single-cell resolution
9:55 AM - 10:15 AM PDT
Claire McWhite (Princeton University)
Talk Title:
Mapping conserved protein complexes across plant species
10:20 AM - 10:40 AM PDT
Shouling Xu (Carnegie Institution for Science)
Talk Title:
From interactome to cell type proteome: applications of proximity labeling mass spectrometry
10:45 AM - 11:30 AM PDT
Panel Discussion: Current and future technologies to help map the location and connection of plant proteins
Protein bodies in maize endosperm, Marisa Otegui
Electron tomographic reconstruction of protein bodies in maize endosperm. Photo Credit: Marisa Otegui
Arabidopsis cotyledon, Flavia Bossi
Reconstruction of an Arabidopsis first leaf from a six-day old seedling. Photo Credit: Flavia Bossi
Developing maize aleurone cell, Marisa Otegui
Electron tomographic reconstruction of a developing maize aleurone cell. Photo Credit: Marisa Otegui
MICHIGAN STATE UNIVERSITY LAND ACKNOWLEDGEMENT
“The Plant Cell Atlas operates predominantly out of Michigan State University. We acknowledge that Michigan State University occupies the ancestral, traditional, and contemporary Lands of the Anishinaabeg – the Three Fires Confederacy of Ojibwe, Odawa, and Potawatomi peoples. We affirm Indigenous sovereignty and hold Michigan State University accountable to the needs of American Indian and Indigenous peoples.”

For questions about the PCA Initiative please contact us at [email protected].

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East Lansing, MI 48824

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Grant #'s: 1916797 & 2052590
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  • Home
  • About
    • Annual Report
    • Newsletter
    • People
    • Motivation
    • Goals
    • Milestones
    • Affilations
    • News >
      • Breaking News
      • Past News
    • Code of Conduct
    • Bylaws
    • Contact
  • Resources
    • Publications >
      • 2022 Plant Physiology Focus Issue
    • Tools and Repositories
    • PCA Videos
  • Events
    • Upcoming Events >
      • Spatial Metabolomics Workshop - March 2025
    • Previous Events
  • Opportunities
    • Job Posts
    • Fellowships
  • Outreach
    • JR Biotek-PCA Best Talk Winner
    • PCA Art Exhibit >
      • About
      • Plant Biotechnology
      • Black Plant Scientists
      • Bowie State University
      • Howard University
      • Morgan State University
    • Futurum Brochure
  • Blog