Objectives

Registration

The goal of the Plant Cell Atlas (PCA) is to bring together a community who will  comprehensively describe plant cell types by integrating high-resolution subcellular and cellular location information of nucleic acids, proteins, and metabolites. In line with achieving this goal, proteomics represents a central facet of the systems-level landscape. Significant advances in mass spectrometry technologies and proteomic workflows now make proteomics notably more accessible for plant science. This PCA workshop aims to bring together world leaders in spatial and quantitative proteomics from plant sciences and beyond to help realize the development of a comprehensive PCA proteome.

Workshop Organizers and Speakers

Registration for the PCA Spatial Proteomics Workshop is now closed.

A Zoom link will be provided to registrants the day before the workshop.

You can find recordings of four of the talks on the Plant Cell Atlas YouTube Channel. Links to the individual talks can be found in the agenda.

Workshop Times
PDT - 8:00am - 11:30am
MDT - 9:00am - 12:30pm
CDT - 10:00am - 1:30pm
EDT - 11:00am - 2:30pm
CEST - 5:00pm - 8:30pm
GMT+8 - 11:00pm - 2:30am (4/9/2021)

Epidermal plasma membrane in green and nuclei (H2B-TFP) in blue, together with chlorophyll autofluorescence in red below in the mesophyll. Photo credit: Kate Harline (Roeder Lab)

Pin1-YFP (yellow) and DR5::RFP (red) in the maize shoot apex. Photo credit: Leiboff Lab

Depth-coded image of microtubules in root hairs of the model grass, Brachypodium distachyon. Photo credit: Elison Blancaflor

 

Agenda

8:00 AM - 8:05 AM PDT

Introduction

8:05 AM - 8:25 AM PDT

Kathryn Lilley (University of Cambridge)
Talk Title: Spatial proteomics methods to map subcellular protein distribution and re-localisation

8:30 AM - 8:50 AM PDT

Ryan Kelly (Brigham Young University)
Talk Title: In-depth and high-spatial-resolution proteome profiling of plant tissues

8:55 AM - 9:15 AM PDT

Julia Mergner (Technical University of Munich)
Talk Title: Protein expression profiling in Arabidopsis tissues

9:20 AM - 9:30 AM PDT

Break

9:30 AM - 9:50 AM PDT

Nikolai Slavov (Northeastern University)
Talk Title: Mapping the transcriptome and proteome of tissues in 3D at single-cell resolution

9:55 AM - 10:15 AM PDT

Claire McWhite (Princeton University)
Talk Title: Mapping conserved protein complexes across plant species

10:20 AM - 10:40 AM PDT

Shouling Xu (Carnegie Institution for Science)
Talk Title: From interactome to cell type proteome: applications of proximity labeling mass spectrometry

10:45 AM - 11:30 AM PDT

Panel Discussion: Current and future technologies to help map the location and connection of plant proteins

Electron tomographic reconstruction of protein bodies in maize endosperm. Photo Credit: Marisa Otegui

Reconstruction of an Arabidopsis first leaf from a six-day old seedling. Photo Credit: Flavia Bossi

Electron tomographic reconstruction of a developing maize aleurone cell. Photo Credit: Marisa Otegui