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Single Cell Sequencing Techniques in Plant Biology
Part 1: From Plants to Data Generation
Virtual Workshop
April 14, 2023
 

Objectives

Registration

The first part of the two-part workshop on Single Cell Sequencing Techniques in Plant Biology will focus on bridging the gap between laboratory work and data generation. If you are starting your journey or already gained experience with single cell transcriptomics, multiomics, or spatial transcriptomics, this will be an excellent place to hear from skilled researchers and industry-leading companies about the key points and techniques necessary for a successful and high-quality dataset. We will discuss experimental setups and procedures, including sample preparation techniques, different assay types and technical considerations.
Registration is closed for the Single Cell Sequencing Techniques in Plant Biology - Part 1 workshop.
Workshop Times
PDT - 8:00am - 12:30pm
MDT - 9:00am - 1:30pm
CDT - 10:00am - 2:30pm
EDT - 11:00am - 3:30pm
CEST - 5:00pm - 9:30pm
GMT+8 - 11:00pm - 3:30am (4/15/2023)
Search for your time zone here.

Recordings of the six of the talks can be found on the PCA YouTube channel.

Workshop Organizers and Speakers

Epidermal plasma membrane in green and nuclei (H2B-TFP) in blue, together with chlorophyll autofluorescence in red below in the mesophyll. Photo credit: Kate Harline (Roeder Lab)
Epidermal plasma membrane in green and nuclei (H2B-TFP) in blue, together with chlorophyll autofluorescence in red below in the mesophyll. Photo credit: Kate Harline (Roeder Lab)
Pin1-YFP (yellow) and DR5::RFP (red) in the maize shoot apex. Photo credit: Leiboff Lab
Pin1-YFP (yellow) and DR5::RFP (red) in the maize shoot apex. Photo credit: Leiboff Lab
Depth-coded image of microtubules in root hairs of the model grass, Brachypodium distachyon. Photo credit: Elison Blancaflor
 

Speakers

  • Stefania Giacomello (Science for Life Laboratory, Sweden)
  • Hilde Nelissen (VIB Center for Plant Systems Biology/Ghent University, Belgium)
  • Trevor Nolan (Duke University, USA)
  • Gergo Palfalvi (Max Planck Institute for Plant Breeding Research, Germany)
  • Egon Ranghini (10X Genomics, USA)
  • Dongbo Shi (University of Potsdam, Germany)
  • Joseph Swift (Salk Institute for Biological Studies, USA)
  • Sandra Thibivilliers (University of Nebraska-Lincoln, USA)
  • Bertrand Yeung (Curio Bioscences, USA)

Agenda

8:00 AM - 8:05 AM PDT
Opening Remarks

Single Cell Sequencing Techniques in the Lab

8:05 AM - 8:20 AM PDT
Trevor Nolan (Duke University, USA)
8:20 AM - 8:35 AM PDT
Gergo Palfalvi (Max Planck Institute for Plant Breeding Research, Germany)
8:35 AM - 8:50 AM PDT
Dongbo Shi (University of Potsdam, Germany)
8:50 PM - 9:25 AM PDT
Panel Discussion
Single Cell Sequencing Techniques in the Lab
9:25 AM - 9:30 AM PDT
Coffee Break

Introduction to Multimodal Measurements

9:30 AM - 9:45 AM PDT
Egon Ranghini (10X Genomics, USA)
9:45 AM - 10:00 AM PDT
Sandra Thibivilliers (University of Nebraska-Lincoln, USA)
10:00 AM - 10:15 AM PDT
Joseph Swift (Salk Institute for Biological Studies, USA)
10:15 AM - 10:50 AM PDT
Panel Discussion
Introduction to Multimodal Measurements

10:50 AM - 11:00 AM PDT
Coffee Break

Spatial Omics in Plant Biology

11:00 AM - 11:15 AM PDT
Bertrand Yeung (Curio Bioscences, USA)
11:15 AM - 11:30 AM PDT
Stefania Giacomello (Science for Life Laboratory, Sweden)
11:30 AM - 11:45 AM PDT
Hilde Nelissen (VIB-PSB/Ghent University, Belgium)
11:45 AM - 12:20 PM PDT
Panel Discussion
Spatial Omics in Plant Biology
12:20 PM - 12:30 PM PDT
Closing Remarks
Protein bodies in maize endosperm, Marisa Otegui
Electron tomographic reconstruction of protein bodies in maize endosperm. Photo Credit: Marisa Otegui
Arabidopsis cotyledon, Flavia Bossi
Reconstruction of an Arabidopsis first leaf from a six-day old seedling. Photo Credit: Flavia Bossi
Developing maize aleurone cell, Marisa Otegui
Electron tomographic reconstruction of a developing maize aleurone cell. Photo Credit: Marisa Otegui
MICHIGAN STATE UNIVERSITY LAND ACKNOWLEDGEMENT
“The Plant Cell Atlas operates predominantly out of Michigan State University. We acknowledge that Michigan State University occupies the ancestral, traditional, and contemporary Lands of the Anishinaabeg – the Three Fires Confederacy of Ojibwe, Odawa, and Potawatomi peoples. We affirm Indigenous sovereignty and hold Michigan State University accountable to the needs of American Indian and Indigenous peoples.”

For questions about the PCA Initiative please contact us at [email protected].

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  • Home
  • About
    • Annual Report
    • Newsletter
    • People
    • Motivation
    • Goals
    • Milestones
    • Affilations
    • News >
      • Breaking News
      • Past News
    • Code of Conduct
    • Bylaws
    • Contact
  • Resources
    • Publications >
      • 2022 Plant Physiology Focus Issue
    • Tools and Repositories
    • PCA Videos
  • Events
    • Upcoming Events >
      • Spatial Metabolomics Workshop - March 2025
    • Previous Events
  • Opportunities
    • Job Posts
    • Fellowships
  • Outreach
    • JR Biotek-PCA Best Talk Winner
    • PCA Art Exhibit >
      • About
      • Plant Biotechnology
      • Black Plant Scientists
      • Bowie State University
      • Howard University
      • Morgan State University
    • Futurum Brochure
  • Blog