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Plant Cell Atlas Multiplex Microscopy Symposium
Virtual Workshop
May 19, 2022

 

Objectives

Registration

The goal of the Plant Cell Atlas (PCA) is to bring together a community who will comprehensively describe plant cell types by integrating high-resolution subcellular and cellular location information of nucleic acids, proteins, and metabolites. In particular, spatial-omics techniques are rapidly advancing and transforming the way we understand complex organisms and their interactions with the environment. One form, termed “multiplex” microscopy, has been increasingly applied in complex tissue systems to understand disease and development. Multiplex microscopy simultaneously and/or sequentially visualizes an extended array of biomolecular probes on single sections/cell monolayers and intact tissues and organisms, thus providing high-dimensional physical mapping of numerous cellular phenotypes and components over heterogeneous tissues and complements other high-resolution omics such as single-cell sequencing. This PCA symposium brings together pioneers, early adopters and commercial experts in a broad array of cutting-edge multiplex imaging and analysis approaches to encourage further development and adoption by the plant science community.


Registration is closed for the PCA Multiplex Microscopy Symposium.

Workshop Times
PDT - 10:30am - 3:05pm
MDT - 11:30am - 4:05pm
CDT - 12:30pm - 5:05pm
EDT - 1:30pm - 6:05pm
CEST - 6:30pm - 8:05pm
GMT+8 - 12:30am - 6:05am (5/20/2022)
Search for your time zone here.

Recordings of the two of the talks can be found on the PCA YouTube channel.

Workshop Organizers and Speakers

Epidermal plasma membrane in green and nuclei (H2B-TFP) in blue, together with chlorophyll autofluorescence in red below in the mesophyll. Photo credit: Kate Harline (Roeder Lab)
Epidermal plasma membrane in green and nuclei (H2B-TFP) in blue, together with chlorophyll autofluorescence in red below in the mesophyll. Photo credit: Kate Harline (Roeder Lab)
Pin1-YFP (yellow) and DR5::RFP (red) in the maize shoot apex. Photo credit: Leiboff Lab
Pin1-YFP (yellow) and DR5::RFP (red) in the maize shoot apex. Photo credit: Leiboff Lab
Depth-coded image of microtubules in root hairs of the model grass, Brachypodium distachyon. Photo credit: Elison Blancaflor
 

Speakers

  • Regan Baird (Visiopharm)
    • Talk Topic: Multiplex Image Processing/Analysis
  • Jiang He (Vizgen)
    • Talk Topic: Multiplexed Error Robust Fluorescence In Situ Hybridization (MERFISH) Method
  • Sizun Jiang (Beth Israel Deaconess Medical Center)
    • Talk Topic: CODEX, Multiplexed Ion Beam Imaging (MIBI)
  • Yangning Lu (Massachusetts Institute of Technology)
    • Talk Topic: Multiplex Expansion Microscopy
  • Lingyan Shi (University of California, San Diego)
    • Talk Topic: Stimulated Raman Scattering (SRS) - Multiplex Metabolism
  • Peng Yin (Harvard University)
    • Talk Topic: DNA-based imaging

Agenda

10:30 AM - 10:35 AM PDT
Opening Remarks

10:35 AM - 11:15 AM PDT
Lingyan Shi (University of California, San Diego)
Talk Topic: Stimulated Raman Scattering (SRS) - Multiplex Metabolism
11:15 AM - 11:55 AM PDT
Peng Yin (Harvard University)
Talk Topic: DNA probes for multiplexed imaging: DNA-Exchange, DNA-PAINT, and SABER

11:55 AM - 12:35 PM PDT

Jiang He (Vizgen)

Talk Topic: Multiplexed Error Robust Fluorescence In Situ Hybridization (MERFISH) Method
12:35 PM - 12:50 PM PDT
Break

12:50 PM - 1:30 PM PDT
Yangning Lu (Massachusetts Institute of Technology)
Talk Topic: Multiplex Expansion Microscopy

1:30 PM - 2:10 PM PDT
Regan Baird (Visiopharm)
Talk Topic: Multiplex Image Processing/Analysis

2:10 PM - 3:00 PM PDT
Sizun Jiang (Beth Israel Deaconess Medical Center)
Talk Topic: CODEX, Multiplexed Ion Beam Imaging (MIBI)

3:00 PM - 3:05 PM PDT
Closing Remarks
Protein bodies in maize endosperm, Marisa Otegui
Electron tomographic reconstruction of protein bodies in maize endosperm. Photo Credit: Marisa Otegui
Arabidopsis cotyledon, Flavia Bossi
Reconstruction of an Arabidopsis first leaf from a six-day old seedling. Photo Credit: Flavia Bossi
Developing maize aleurone cell, Marisa Otegui
Electron tomographic reconstruction of a developing maize aleurone cell. Photo Credit: Marisa Otegui
MICHIGAN STATE UNIVERSITY LAND ACKNOWLEDGEMENT
“The Plant Cell Atlas operates predominantly out of Michigan State University. We acknowledge that Michigan State University occupies the ancestral, traditional, and contemporary Lands of the Anishinaabeg – the Three Fires Confederacy of Ojibwe, Odawa, and Potawatomi peoples. We affirm Indigenous sovereignty and hold Michigan State University accountable to the needs of American Indian and Indigenous peoples.”

For questions about the PCA Initiative please contact us at [email protected].

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East Lansing, MI 48824

National Science Foundation logo. A blue globe with
Grant #'s: 1916797 & 2052590
Plant Resilience Institute at Michigan State University logo
Carnegie Science logo on the left. The logo depicts a circle with a sytlized dark blue starry sky at the top, a brown ammonite fossile on the bottem left, and a stylized green leaf on the right. Text to the right of the logo states
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  • Home
  • About
    • Annual Report
    • Newsletter
    • People
    • Motivation
    • Goals
    • Milestones
    • Affilations
    • News >
      • Breaking News
      • Past News
    • Code of Conduct
    • Bylaws
    • Contact
  • Resources
    • Publications >
      • 2022 Plant Physiology Focus Issue
    • Tools and Repositories
    • PCA Videos
  • Events
    • Upcoming Events >
      • Spatial Metabolomics Workshop - March 2025
    • Previous Events
  • Opportunities
    • Job Posts
    • Fellowships
  • Outreach
    • JR Biotek-PCA Best Talk Winner
    • PCA Art Exhibit >
      • About
      • Plant Biotechnology
      • Black Plant Scientists
      • Bowie State University
      • Howard University
      • Morgan State University
    • Futurum Brochure
  • Blog