Objectives

Registration

The goal of the Plant Cell Atlas (PCA) is to bring together a community who will comprehensively describe plant cell types by integrating high-resolution subcellular and cellular location information of nucleic acids, proteins, and metabolites. In particular, spatial-omics techniques are rapidly advancing and transforming the way we understand complex organisms and their interactions with the environment. One form, termed “multiplex” microscopy, has been increasingly applied in complex tissue systems to understand disease and development. Multiplex microscopy simultaneously and/or sequentially visualizes an extended array of biomolecular probes on single sections/cell monolayers and intact tissues and organisms, thus providing high-dimensional physical mapping of numerous cellular phenotypes and components over heterogeneous tissues and complements other high-resolution omics such as single-cell sequencing. This PCA symposium brings together pioneers, early adopters and commercial experts in a broad array of cutting-edge multiplex imaging and analysis approaches to encourage further development and adoption by the plant science community.

Registration is closed for the PCA Multiplex Microscopy Symposium.

Workshop Times
PDT - 10:30am - 3:05pm
MDT - 11:30am - 4:05pm
CDT - 12:30pm - 5:05pm
EDT - 1:30pm - 6:05pm
CEST - 6:30pm - 8:05pm
GMT+8 - 12:30am - 6:05am (5/20/2022)
Search for your time zone here.

Recordings of the two of the talks can be found on the PCA YouTube channel.

Workshop Organizers and Speakers

Epidermal plasma membrane in green and nuclei (H2B-TFP) in blue, together with chlorophyll autofluorescence in red below in the mesophyll. Photo credit: Kate Harline (Roeder Lab)

Pin1-YFP (yellow) and DR5::RFP (red) in the maize shoot apex. Photo credit: Leiboff Lab

Depth-coded image of microtubules in root hairs of the model grass, Brachypodium distachyon. Photo credit: Elison Blancaflor

 

Speakers

• Regan Baird (Visiopharm)
  • Talk Topic: Multiplex Image Processing/Analysis
• Jiang He (Vizgen)
  • Talk Topic: Multiplexed Error Robust Fluorescence In Situ Hybridization (MERFISH) Method
    
• Sizun Jiang (Beth Israel Deaconess Medical Center)
  
  • Talk Topic: CODEX, Multiplexed Ion Beam Imaging (MIBI)
    
• Yangning Lu (Massachusetts Institute of Technology)
  • Talk Topic: Multiplex Expansion Microscopy
    
• Lingyan Shi (University of California, San Diego)
  • Talk Topic: Stimulated Raman Scattering (SRS) - Multiplex Metabolism
• Peng Yin (Harvard University)
  • Talk Topic: DNA-based imaging

Agenda

10:30 AM - 10:35 AM PDT

Opening Remarks

10:35 AM - 11:15 AM PDT

Lingyan Shi (University of California, San Diego)
Talk Topic: Stimulated Raman Scattering (SRS) - Multiplex Metabolism

11:15 AM - 11:55 AM PDT

Peng Yin (Harvard University)
Talk Topic: DNA probes for multiplexed imaging: DNA-Exchange, DNA-PAINT, and SABER

11:55 AM - 12:35 PM PDT

Jiang He (Vizgen)
Talk Topic: Multiplexed Error Robust Fluorescence In Situ Hybridization (MERFISH) Method

12:35 PM - 12:50 PM PDT

Break

12:50 PM - 1:30 PM PDT

Yangning Lu (Massachusetts Institute of Technology)
Talk Topic: Multiplex Expansion Microscopy

1:30 PM - 2:10 PM PDT

Regan Baird (Visiopharm)
Talk Topic: Multiplex Image Processing/Analysis

2:10 PM - 3:00 PM PDT

Sizun Jiang (Beth Israel Deaconess Medical Center)
Talk Topic: CODEX, Multiplexed Ion Beam Imaging (MIBI)

3:00 PM - 3:05 PM PDT

Closing Remarks

Electron tomographic reconstruction of protein bodies in maize endosperm. Photo Credit: Marisa Otegui

Reconstruction of an Arabidopsis first leaf from a six-day old seedling. Photo Credit: Flavia Bossi

Electron tomographic reconstruction of a developing maize aleurone cell. Photo Credit: Marisa Otegui