Objectives

The goal of the Plant Cell Atlas (PCA) is to bring together a community who will comprehensively describe plant cell types by integrating high-resolution subcellular and cellular location information of nucleic acids, proteins, and metabolites.

Symposium Goals:
1) Share the most exciting scientific and technological breakthroughs in plant single cell science.
2) Create a forum for people interested in plant single cell approaches to meet and network.
3) Discuss community building and how PCA relevant data could be analyzed, annotated, and accessed.

Each day will consist of three oral sessions and two poster sessions. Each oral session will feature one invited speaker and 2 speakers selected from abstract submissions. Each oral session will also include time to discuss PCA community building and data handling. Further details of the agenda will be posted here in the upcoming months.

Recordings of the five of the talks can be found on the PCA YouTube channel.
Symposium Organizers and Speakers
​
PCA Code of Conduct
Designated Safety Officers:

• Sue Rhee - srhee@carnegiescience.edu 
• Nicholas Provart - nicholas.provart@utoronto.ca

 

Invited Speakers

• Federica Brandizzi (Michigan State University, USA)
• Kevin Cox, Jr (Donald Danforth Plant Science Center, USA)
• Stefania Giacomello (SciLifeLab - KTH, Sweden)
• Yvon Jaillais (ENS Lyon, France)
• Mathew Lewsey (La Trobe University, Australia)
• Irene Papatheodorou (European Molecular Biology Lab-EBI, UK)

Projection of a confocal image stack showing the edge of a cotyledon. Pavement cells and guard cells are visible. Photo credit: D. Ehrhardt

Arabidopsis trichome with the nucleus marked by two different histone H3 proteins fused to fluorescent reporters. Cover of TPJ, vol 83, issue 1. Photo credit: S. Otero

Cross section of a DAPI stained Populous tremula dormant leaf bud. Photo credit: S. Giacomello and B. Terebieniec

 

Registration

Register Here

Registration deadline: None
Location: Virtual
Cost: Free
Dates: Dec 9 & 10, 2021
Times: 7:00am - 2:00pm PST

Time converter at worldtimebuddy.com

 

Abstract Submission

We are no longer accepting abstracts.

Abstract Submission Deadline - Talks: closed
Abstract Submission Deadline - Posters: closed
Poster File Submission Deadline - Wave 1: December 1, 2021 
Poster File Submission Deadline - Wave 2: December 6, 2021 (more info below)

There is no fee to submit an abstract. NOTE: You still need to register for the meeting through the registration link above even if you submit an abstract.

Abstracts should be no longer than 1 page and submitted as a PDF or Word document. Please include:

• Title
• Author(s) - presenter name in bold
• Affiliation
• Abstract

Those whose abstracts have been selected for a talk will be notified by November 9, 2021. Those whose abstracts were submitted by October 27, 2021 (Wave 1) and have been selected for a poster will be notified by November 23, 2021. Those whose abstracts were submitted by November 22, 2021 (Wave 2) and have been selected for a poster will be notified by November 29, 2021.

Epidermal plasma membrane in green and nuclei (H2B-TFP) in blue, together with chlorophyll autofluorescence in red below in the mesophyll. Photo credit: Kate Harline (Roeder Lab)

Pin1-YFP (yellow) and DR5::RFP (red) in the maize shoot apex. Photo credit: Leiboff Lab

Depth-coded image of microtubules in root hairs of the model grass, Brachypodium distachyon. Photo credit: Elison Blancaflor

 

Schedule

DAY 1
December 9, 2021

Oral Session 1
Moderator: Carol Huang
7:00 AM - 8:30 AM PST

7:00 AM - 7:20 AM PST

Yvon Jaillais (ENS Lyon, France)
Talk Title: Anionic phospholipids across scales: from plasma membrane nanodomains to plant development

7:30 AM - 7:40 AM PST

Hilde Nelissen (VIB-Ghent University, Belgium)
Talk Title: An in situ sequencing approach maps PLASTOCHRON1 at the boundary between indeterminate and determinate cells

7:45 AM - 7:55 AM PST

Gergo Palfalvi (National Institute for Basic Biology, Japan)
Talk Title: Evolution and development of carnivorous plant leaves

8:00 AM - 8:30 AM PST

Community Discussion 1
What data analysis tools and annotation standards are needed to support and centralize the community's research?

Poster Session 1
8:30 AM - 9:30 AM PST

8:30 AM - 8:50 AM PST

Section 1A

8:50 AM - 9:10 AM PST

Section 1B

9:10 AM - 9:30 AM PST

Section 1C

Break
9:30 AM - 9:40 AM PST

Oral Session 2
Moderators: Benjamin Cole & Gergo Palfavi
9:40 AM - 11:10 AM PST

9:40 AM - 10:00 AM PST

Stefania Giacomello (SciLifeLab - KTH, Sweden)
Talk Title: Unveiling spatial host-microbiome interactions by applying Spatial metaTranscriptomics

10:10 AM - 10:20 AM PST

Lachezar Nikolov (University of California, Los Angeles, USA)
Talk Title: Tabula floris - panoramic view of the cell types of the flower

10:25 AM - 10:35 AM PST

Xiaosa Xu (Cold Spring Harbor Laboratory, USA)
Talk Title: A high-resolution single-cell atlas of shoot meristems at cross-species and multimodal levels

10:40 AM - 11:10 AM PST

Community Discussion 2
What types of resources, including computational expertise and cyber-infrastructure, will the community need to best advance the science and meet its goals for broader impacts?

Lunch Break
11:10 AM - 11:50 AM PST

Poster Session 2
11:50 AM - 12:30 PM PST

11:50 AM - 12:10 PM PST

Section 2A

12:10 AM - 12:30 PM PST

Section 2B

Oral Session 3
​Moderator: Samuel Leiboff & Rachel Shahan
12:30 PM - 2:00 PM PST

12:30 PM - 12:50 PM PST

Mathew Lewsey (La Trobe University, Australia)
Talk Title: Gene regulatory dynamics of germinating from bulk tissue to single-cell resolution

1:00 PM - 1:10 PM PST

Marc Libault (University of Nebraska-Lincoln, USA)
Talk Title: Single-nucleus RNA and ATAC sequencing reveals the impact of chromatin accessibility on gene expression in Arabidopsis roots at the single-cell level

1:15 PM - 1:25 PM PST

Bruno Guillotin (New York University, USA)
Talk Title: Mapping cellular divergence in crop plants via comparative single-cell and nuclei analysis

1:30 PM - 2:00 PM PST

Community Discussion 3
What novel modes of organization and engagement will the community explore to catalyze new ideas, research directions, and discoveries in a time of rapid change?

DAY 2
December 10, 2021

Oral Session 4
​Moderator: Kaisa Kajala & Alex Borowsky
7:00 AM - 8:30 AM PST

7:00 AM - 7:20 AM PST

Irene Papatheodorou + Nancy George (European Molecular Biology Lab-EBI, UK)
Talk Title: Single Cell Expression Atlas: Archiving and Visualisation of Plant Single Cell Data

7:30 AM - 7:40 AM PST

Vimal Kumar Balasubramanian (Pacific Northwest National Laboratory, USA)
​Talk Title: Integration of Cell Type-Specific Omics Analysis Towards a Spatiotemporal Understanding of Molecular Responses to Abiotic Stresses in Poplar

7:45 AM - 7:55 AM PST

Camilla Ferrari (VIB-Ghent University, Belgium)
Talk Title: Identification of cell-type specific gene regulatory networks in plants using MINI-EX, a Motif-Informed Network Inference method based on single-cell EXpression data

8:00 AM - 8:30 AM PST

Community Discussion 4
How will the community ensure that individuals and groups who are not regular participants due to disciplinary barriers, cultural differences, or resource limitations are included in the PCA?

Poster Session 3
8:30 AM - 9:30 AM PST

8:30 AM - 8:50 AM PST

Section 3A

8:50 AM - 9:10 AM PST

Section 3B

9:10 AM - 9:30 AM PST

Section 3C

Break
9:30 AM - 9:40 AM PST

Oral Session 5
Moderators: Shouling Xu & Christopher Anderton
9:40 AM - 11:10 AM PST

9:40 AM - 10:00 AM PST

Kevin Cox, Jr (Donald Danforth Plant Science Center, USA)
Talk Title: Integrating spatial transcriptomics and imaging in plants

10:10 AM - 10:20 AM PST

Margot Bezrutczyk (Lawrence Berkeley National Lab, USA)
Talk Title: Mapping plant-mycorrhizal interactions with spatial transcriptomics and single-nuclei sequencing

10:25 AM - 10:35 AM PST

Maite Colinas (Max Planck Institute for Chemical Ecology, Germany)
Talk Title: Cell type specific responses to ‘friend or foe’: a proteomics-based approach

10:40 AM - 11:10 AM PST

Community Discussion 5
In what ways will the community recruit, train, and nurture new talent to seed paradigm-shifting discoveries in the future?

Lunch Break
11:10 AM - 11:50 AM PST

Poster Session 4
11:50 AM - 12:30 PM PST

11:50 AM - 12:10 PM PST

Section 4A

12:10 AM - 12:30 PM PST

Section 4B

Oral Session 6
Moderator: Marc Libault
12:30 PM - 2:00 PM PST

12:30 PM - 12:50 PM PST

Federica Brandizzi (Michigan State University, USA)
Talk Title: The plant endoplasmic reticulum at the interface with other organelles: How and What for

1:00 PM - 1:10 PM PST

Constance Le Gloanec (University of Montreal, Canada)
Talk Title: Cells switch from stochastic to predictable behaviors during leaf development in Arabidopsis

1:15 PM - 1:25 PM PST

Michael Taylor (Pacific Northwest National Laboratory, USA)
Talk  Title: Development of targeted single cell analysis and metabolic imaging of plants using high spatial resolution laser ablation electrospray ionization mass spectrometry

1:30 PM - 2:00 PM PST

Community Discussion 6
How will the outcomes of the community's efforts serve the wider scientific community?

Electron tomographic reconstruction of protein bodies in maize endosperm. Photo Credit: Marisa Otegui

Reconstruction of an Arabidopsis first leaf from a six-day old seedling. Photo Credit: Flavia Bossi

Electron tomographic reconstruction of a developing maize aleurone cell. Photo Credit: Marisa Otegui